Publications

Talò G, Turrisi C, Arrigoni C, Recordati C, Gerges I, Tamplenizza M, Cappelluti A, Riboldi SA, Moretti M Industrialization of a perfusion bioreactor: prime example of a non-straightforward process. J Tissue Eng Regen Med. 2017 May 16. doi: 10.1002/term.2480. [Epub ahead of print]
 
Bianchi E, Piergiovanni M, Arrigoni C, Fukuda J, Gautieri A, Moretti M, Dubini G. Herringbone-like hydrodynamic structures in microchannels: A CFD model to evaluate the enhancement of surface binding. Med Eng Phys. 2017 Oct;48:62-67. doi: 10.1016/j.medengphy.2017.07.003.
 
Arrigoni C, Gilardi M, Bersini S, Candrian C, Moretti M. Bioprinting and Organ-on-Chip Applications Towards Personalized Medicine for Bone Diseases. Stem Cell Rev. 2017 Jun;13(3):407-417. doi: 10.1007/s12015-017-9741-5.
 
Ugolini GS, Visone R, Redaelli A, Moretti M, Rasponi M. Generating Multicompartmental 3D Biological Constructs Interfaced through Sequential Injections in Microfluidic Devices. Adv Healthc Mater. 2017 May;6(10). doi: 10.1002/adhm.201601170.
 
Bottagisio M, Lopa S, Granata V, Talò G, Bazzocchi C, Moretti M, Barbara Lovati A Different combinations of growth factors for the tenogenic differentiation of bone marrow mesenchymal stem cells in monolayer culture and in fibrin-based three-dimensional constructs. Differentiation. 2017 May - Jun;95:44-53. doi: 10.1016/j.diff.2017.03.001.
 
Enomoto J, Kageyama T, Osaki T, Bonalumi F, Marchese F, Gautieri A, Bianchi E, Dubini G, Arrigoni C, Moretti M, Fukuda J Catch-and-Release of Target Cells Using Aptamer-Conjugated Electroactive Zwitterionic Oligopeptide SAM. Sci Rep. 2017 Mar 7;7:43375. doi: 10.1038/srep43375
 
Zhang YS, Arneri A, Bersini S, Shin SR, Zhu K, Goli-Malekabadi Z, Aleman J, Colosi C, Busignani F, Dell'Erba V, Bishop C, Shupe T, Demarchi D, Moretti M, Rasponi M, Dokmeci MR, Atala A, Khademhosseini A. Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip. Biomaterials. 2016 Dec;110:45-59. doi: 10.1016/j.biomaterials.2016.09.003.
 
Mayer N, Lopa S, Talò G, Lovati AB, Pasdeloup M, Riboldi SA, Moretti M, Mallein-Gerin F. Interstitial Perfusion Culture with Specific Soluble Factors Inhibits Type I Collagen Production from Human Osteoarthritic Chondrocytes in Clinical-Grade Collagen Sponges. PLoS One. 2016 Sep 1;11(9):e0161479. doi: 10.1371/journal.pone.0161479.
 
Arrigoni C, Bersini S, Gilardi M, Moretti M. In Vitro Co-Culture Models of Breast Cancer Metastatic Progression towards Bone. Int J Mol Sci. 2016 Aug 25;17(9). pii: E1405. Review PMID: 27571063
 
Visone R, Gilardi M, Marsano A, Rasponi M, Bersini S, Moretti M. Cardiac Meets Skeletal: What's New in Microfluidic Models for Muscle Tissue Engineering. Molecules. 2016 Aug 26;21(9). pii: E1128. Review. PMID:27571058
 
Bersini S, Yazdi IK, Talò G, Shin SR, Moretti M, Khademhosseini A. Cell-microenvironment interactions and architectures in microvascular systems. Biotechnol Adv. 2016 Nov 1;34(6):1113-30. doi: 10.1016/j.biotechadv.2016.07.002. Epub 2016 Jul 11. Review.PMID:27417066
 
Bottagisio M, Pellegata AF, Boschetti F, Ferroni M, Moretti M, Lovati AB. A new strategy for the decellularisation of large equine tendons as biocompatible tendon substitutes. Eur Cell Mater. 2016 Jul 8;32:58-73. PMID:27386840
 
Lopa S, Ceriani C, Cecchinato R, Zagra L, Moretti M, Colombini A. Stability of housekeeping genes in human intervertebral disc, endplate and articular cartilage cells in multiple conditions for reliable transcriptional analysis.
Eur Cell Mater. 2016 May 27;31:395-406. PMID:2723266
 
Arrigoni C, Bongio M, Talò G, Bersini S, Enomoto J, Fukuda J, Moretti M. Rational Design of Prevascularized Large 3D Tissue Constructs Using Computational Simulations and Biofabrication of Geometrically Controlled Microvessels. Adv Healthc Mater. 2016 Jul;5(13):1617-26. doi: 10.1002/adhm.201500958. Epub 2016 May 18. PMID:27191352
 
 
Gilardi M, Bersini S, Calleja AB, Kamm RD, Vanoni M, Moretti M. PO-12 - The key role of talin-1 in cancer cell extravasation dissected through human vascularized 3D microfluidic model. Thromb Res. 2016 Apr;140 Suppl 1:S180-1. doi: 10.1016/S0049-3848(16)30145-1. Epub 2016 Apr 8.PMID:27161700
 
Bersini S, Arrigoni C, Lopa S, Bongio M, Martin I, Moretti M. Engineered miniaturized models of musculoskeletal diseases. Drug Discov Today. 2016 Apr 28. pii: S1359-6446(16)30120-9. doi: 10.1016/j.drudis.2016.04.015. [Epub ahead of print] Review.PMID:27132520
 
Bongio M, Lopa S, Gilardi M, Bersini S, Moretti M. A 3D vascularized bone remodeling model combining osteoblasts and osteoclasts in a CaP nanoparticle-enriched matrix. Nanomedicine (Lond). 2016 May;11(9):1073-91. doi: 10.2217/nnm-2015-0021. Epub 2016 Apr 14.PMID:27078586
 
Lovati AB, Lopa S, Recordati C, Talò G, Turrisi C, Bottagisio M, Losa M, Scanziani E, Moretti M. In Vivo Bone Formation Within Engineered Hydroxyapatite Scaffolds in a Sheep Model. Calcif Tissue Int. 2016 Aug;99(2):209-23. doi: 10.1007/s00223-016-0140-8. Epub 2016 Apr 13.PMID: 27075029
 
Lovati AB, Romanò CL, Bottagisio M, Monti L, De Vecchi E, Previdi S, Accetta R, Drago L. Modeling Staphylococcus epidermidis-induced non-unions: subclinical and clinical evidence in rats. PLoS One. 2016 Jan 21;11(1):e0147447. doi: 10.1371/journal.pone.0147447. eCollection 2016.
 
Lovati AB, Bottagisio M, Moretti M.  Decellularized and Engineered Tendons as Biological Substitutes: A Critical Review. Stem Cells Int. 2016;2016:7276150. doi: 10.1155/2016/7276150. Epub 2016 Jan 6. Review
 

Lovati AB, Lopa S, Recordati C, Talò G, Turrisi C, Bottagisio M, Losa M, Scanziani E, Moretti M.In Vivo Bone Formation Within Engineered Hydroxyapatite Scaffolds in a Sheep Model. Calcif Tissue Int. 2016 Apr 13. [Epub ahead of print]
 

Bersini S, Gilardi M, Arrigoni C, Talò G, Zamai M, Zagra L, Caiolfa V, Moretti M. Human in vitro 3D co-culture model to engineer vascularized bone-mimicking tissues combining computational tools and statistical experimental approach. Biomaterials. 2015 Oct 24;76:157-172. doi: 10.1016/j.biomaterials.2015.10.057.
 
Raimondi MT, Bertoldi S, Caddeo S, Faré S, Arrigoni C and Moretti M. The effect of polyurethane scaffolds surface treatments on the adhesion of
chondrocytes subjected to interstitial perfusion culture.
Tissue Eng Regen Med in press.
 
Bersini S, Moretti M. 3D functional and perfusable microvascular networks for organotypic microfluidic models. J Mater Sci Mater Med. 2015 May;26(5):5520
 
Lopa S, Piraino F, Kemp RJ, Di Caro C, Lovati AB, Di Giancamillo A, Moroni L, Peretti GM, Rasponi M, Moretti M. Fabrication of multi-well chips for spheroid cultures and implantable constructs through rapid prototyping techniques. Biotechnol Bioeng. 2015 Feb. doi: 10.1002/bit.25557
 
Jeon JS, Bersini S, Gilardi M, Dubini G, Charest JL, Moretti M*, Kamm RD*. Human 3D vascularized organotypic microfluidic assays to study breast cancer cell extravasation. PNAS. 2015 Jan;112(1):214-9.
 
Bottagisio M, Lovati AB, Lopa S, Moretti M. Osteogenic differentiation of human and ovine bone marrow stromal cells in response to β-glycerophosphate and monosodium phosphate. Cellular Reprogramming, 2015; [in press]
 
Colombini A, Lopa S, Ceriani C, Lovati AB, Croiset SJ, Di Giancamillo A, Lombardi G, Banfi G, Moretti M. In vitro characterization and in vivo behavior of human nucleus pulposus and annulus fibrosus cells in clinical-grade fibrin and collagen-enriched fibrin gels. Tissue Eng Part A. 2015 Feb;21(3-4):793-802.
 
Lovati AB, Lopa S, Talò G, Previdi S, Recordati C, Mercuri D, Segatti F, Zagra L, Moretti M. In vivo evaluation of bone deposition in macroporous titanium implants loaded with mesenchymal stem cells and strontium-enriched hydrogel. J Biomed Mater Res B Appl Biomater. 2015 Feb;103(2):448-56.
 
Lovati AB, Pozzi A, Bongio M, Recordati C, Berzero G, Moretti M. A comparative study of diagnostic and imaging techniques for osteoarthritis of the trapezium. Rheumatology (Oxford). 2015 Jan;54(1):96-103.
 
Colombini A, Ceriani C, Banfi G, Brayda-Bruno M, Moretti M. Fibrin in intervertebral disc tissue engineering. Tissue Eng Part B Rev. 2014 Dec;20(6):713-21.
 
Occhetta P, Visone R, Russo L, Cipolla L, Moretti M, Rasponi M. VA-086 methacrylate gelatine photopolymerizable hydrogels: A parametric study for highly biocompatible 3D cell embedding. J Biomed Mater Res A. 2014 Oct. doi: 10.1002/jbm.a.35346.
 
Arrigoni C, De Luca P, Gilardi M, Previdi S, Broggini M, Moretti M. Direct but not indirect co-culture with osteogenically differentiated human bone marrow stromal cells increases RANKL/OPG ratio in human breast cancer cells generating bone metastases. Mol Cancer. 2014 Oct;13:238.
 
Lopa S, Madry H. Bioinspired scaffolds for osteochondral regeneration. Tissue Eng Part A. 2014 Aug;20(15-16):2052-76.
 
Bersini S, Jeon JS, Moretti M, Kamm RD. In vitro models of the metastatic cascade: from local invasion to extravasation. Drug Discov Today. 2014 Jun;19(6):735-42.
 
de Girolamo L, Stanco D, Galliera E, Viganò M, Lovati AB, Marazzi MG, Romeo P, Sansone V. Soft-focused extracorporeal shock waves increase the expression of tendon-specific markers and the release of anti-inflammatory cytokines in an adherent culture model of primary human tendon cells. Ultrasound Med Biol. 2014 Jun;40(6):1204-15.
 
Jeon JS, Bersini S, Whisler JA, Chen MB, Dubini G, Charest JL, Moretti M, Kamm RD. Generation of 3D functional microvascular networks with human mesenchymal stem cells in microfluidic systems. Integr Biol (Camb). 2014 May;6(5):555-63.
 
Lopa S, Colombini A, Stanco D, de Girolamo L, Sansone V, Moretti M. Donor-matched mesenchymal stem cells from knee infrapatellar and subcutaneous adipose tissue of osteoarthritic donors display differential chondrogenic and osteogenic commitment. Eur Cell Mater. 2014 Apr 23;27:298-311.
 
Lovati AB, Romanò CL, Monti L, Vassena C, Previdi S, Drago L. Does PGE₁ vasodilator prevent orthopaedic implant-related infection in diabetes? Preliminary results in a mouse model. PLoS One. 2014 Apr;9(4):e94758.
 
Bersini S, Jeon JS, Dubini G, Arrigoni C, Chung S, Charest JL, Moretti M*, Kamm RD*. A microfluidic 3D in vitro model for specificity of breast cancer metastasis to bone. Biomaterials. 2014 Mar;35(8):2454-61.
 
Talò G, Turrisi C, Lovati AB, Moretti M. Bioreactors for musculoskeletal tissue engineering. Journal of Sports Traumatology. 2014 Mar;31(1):13-22.
 
Lopa S, Mercuri D, Colombini A, De Conti G, Segatti F, Zagra L, Moretti M. Orthopedic bioactive implants: hydrogel enrichment of macroporous titanium for the delivery of mesenchymal stem cells and strontium. J Biomed Mater Res A. 2013 Dec;101(12):3396-403.
 
Lovati AB, Drago L, Monti L, De Vecchi E, Previdi S, Banfi G, Romanò CL. Diabetic mouse model of orthopaedic implant-related Staphylococcus aureus infection. PLoS One. 2013 Jun 20;8(6):e67628.
 
Occhetta P, Sadr N, Piraino F, Redaelli A, Moretti M*, Rasponi M*. Fabrication of 3D cell-laden hydrogel microstructures through photo-mold patterning. Biofabrication. 2013 Sep;5(3):035002.
 
Laganà M, Arrigoni C, Lopa S, Sansone V, Zagra L, Moretti M*, Raimondi MT*. Characterization of articular chondrocytes isolated from 211 osteoarthritic patients. Cell Tissue Bank. 2014 Mar;15(1):59-66.
 
Lopa S, Colombini A, Sansone V, Preis FW, Moretti M. Influence on chondrogenesis of human osteoarthritic chondrocytes in co-culture with donor-matched mesenchymal stem cells from infrapatellar fat pad and subcutaneous adipose tissue. Int J Immunopathol Pharmacol. 2013 Jan-Mar;26(1 Suppl):23-31.
 
Sabatino MA, Santoro R, Gueven S, Jaquiery C, Wendt DJ, Martin I, Moretti M, Barbero A. Cartilage graft engineering by co-culturing primary human articular chondrocytes with human bone marrow stromal cells. J Tissue Eng Regen Med. 2012 Dec. doi: 10.1002/term.1661.
 
Lovati AB, Corradetti B, Cremonesi F, Bizzaro D, Consiglio AL. Tenogenic differentiation of equine mesenchymal progenitor cells under indirect co-culture. Int J Artif Organs. 2012 Nov;35(11):996-1005.
 
Sadr N, Zhu M, Osaki T, Kakegawa T, Yang Y, Moretti M, Fukuda J, Khademhosseini A. SAM-based cell transfer to photopatterned hydrogels for microengineering vascular-like structures. Biomaterials. 2011 Oct;32(30):7479-90.
 
 
Lopa S, Colombini A, de Girolamo L, Sansone V, Moretti M. New Strategies in Cartilage Tissue Engineering for Osteoarthritic Patients: Infrapatellar Fat Pad as an Alternative Source of Progenitor Cells. Journal of Biomaterials and Tissue Engineering, 2011 Jun;1(1):40-48.
 
Pavesi A, Piraino F, Fiore GB, Farino KM, Moretti M*, Rasponi M*. How to embed three-dimensional flexible electrodes in microfluidic devices for cell culture applications. Lab Chip. 2011 May;11(9):1593-5.
 
Lovati AB, Vianello E, Talò G, Recordati C, Bonizzi L, Galliera E, Broggini M, Moretti M. Biodegradable microcarriers as cell delivery vehicle for in vivo transplantation and magnetic resonance monitoring. J Biol Regul Homeost Agents. 2011 Apr-Jun;25(2 Suppl):S63-74.
 
Lovati AB, Corradetti B, Lange Consiglio A, Recordati C, Bonacina E, Bizzaro D, Cremonesi F. Characterization and differentiation of equine tendon-derived progenitor cells. J Biol Regul Homeost Agents. 2011 Apr-Jun;25(2 Suppl):S75-84.
 
Raimondi MT, Bonacina E, Candiani G, Laganà M, Rolando E, Talò G, Pezzoli D, D'Anchise R, Pietrabissa R, Moretti M. Comparative chondrogenesis of human cells in a 3D integrated experimental-computational mechanobiology model. Biomech Model Mechanobiol. 2011 Apr;10(2):259-68.
 
Rasponi M, Piraino F, Sadr N, Laganà M, Redaelli A, Moretti M. Reliable magnetic reversible assembly of complex microfluidic devices: fabrication, characterization, and biological validation. Microfluidics and Nanofluidics, 2011 May;10(5):1097-1107.
 
Cioffi M*, Moretti M*, Manbachi A, Chung BG, Khademhosseini A, Dubini G. A computational and experimental study inside microfluidic systems: the role of shear stress and flow recirculation in cell docking. Biomed Microdevices. 2010 Aug;12(4):619-26.
 
Valonen PK, Moutos FT, Kusanagi A, Moretti MG, Diekman BO, Welter JF, Caplan AI, Guilak F, Freed LE. In vitro generation of mechanically functional cartilage grafts based on adult human stem cells and 3D-woven poly(epsilon-caprolactone) scaffolds. Biomaterials. 2010 Mar;31(8):2193-200.
 
Cheng M*, Moretti M*, Engelmayr GC, Freed LE. Insulin-like growth factor-I and slow, bi-directional perfusion enhance the formation of tissue-engineered cardiac grafts. Tissue Eng Part A. 2009 Mar;15(3):645-53.
 
Moretti M, Freed LE, Padera RF, Laganà K, Boschetti F, Raimondi MT. An integrated experimental-computational approach for the study of engineered cartilage constructs subjected to combined regimens of hydrostatic pressure and interstitial perfusion. Biomed Mater Eng. 2008;18(4-5):273-8.
 
Manbachi A, Shrivastava S, Cioffi M, Chung BG, Moretti M, Demirci U, Yliperttula M, Khademhosseini A. Microcirculation within grooved substrates regulates cell positioning and cell docking inside microfluidic channels. Lab Chip. 2008 May;8(5):747-54.
 
Laganà K, Moretti M, Dubini G, Raimondi MT. A new bioreactor for the controlled application of complex mechanical stimuli for cartilage tissue engineering. Proc Inst Mech Eng H. 2008 Jul;222(5):705-15.
 
Raimondi MT, Candiani G, Cabras M, Cioffi M, Laganà K, Moretti M, Pietrabissa R. Engineered cartilage constructs subject to very low regimens of interstitial perfusion. Biorheology. 2008;45(3-4):471-8.
 
Cheng M, Park H, Engelmayr GC, Moretti M, Freed LE. Effects of regulatory factors on engineered cardiac tissue in vitro. Tissue Eng. 2007 Nov;13(11):2709-19.
 
Raimondi MT, Moretti M, Cioffi M, Giordano C, Boschetti F, Laganà K, Pietrabissa R. The effect of hydrodynamic shear on 3D engineered chondrocyte systems subject to direct perfusion. Biorheology. 2006;43(3-4):215-22.
 
 
Moretti M, Wendt D, Dickinson SC, Sims TJ, Hollander AP, Kelly DJ, Prendergast PJ, Heberer M, Martin I. Effects of in vitro preculture on in vivo development of human engineered cartilage in an ectopic model. Tissue Eng. 2005 Sep-Oct;11(9-10):1421-8.
 
Moretti M, Wendt D, Schaefer D, Jakob M, Hunziker EB, Heberer M, Martin I. Structural characterization and reliable biomechanical assessment of integrative cartilage repair. J Biomech. 2005 Sep;38(9):1846-54.
 
Schaefer DB, Wendt D, Moretti M, Jakob M, Jay GD, Heberer M, Martin I. Lubricin reduces cartilage--cartilage integration. Biorheology. 2004;41(3-4):503-8.
 
Moretti M, Prina-Mello A, Reid AJ, Barron V, Prendergast PJ. Endothelial cell alignment on cyclically-stretched silicone surfaces. J Mater Sci Mater Med. 2004 Oct;15(10):1159-64.
 
Additional Publications
Moretti M, Arrigoni C, Lovati AB, Talò G. Bioinspired Cell Culture Bioreactors. Handbook of Biomimetics and Bioinspiration. World Scientific Publishing Co. 2013
 
 
Moretti M, Cheng MY, Freed LE. Oscillating Cell Culture Bioreactor. N.A.S.A. Medical Design Briefs 2010, Mar;25-26.
http://www.techbriefs.com/component/content/article/10-ntb/tech-briefs/bio-medical/7567
 
Moretti M (contributor). Roadmaps in Nanomedicine towards 2020. Joint European Comission / ETPNanomedicine report 2009.
http://www.etp-nanomedicine.eu/public/press-documents/publications/etpn-publications/091022_ETPN_Report_2009.pdf


NEWS / EVENTS

25/06/2012 Meet us at ESB 2012 in Lisbon (1-4 Jul)!
20/05/2012 Silvia Lopa will spend one month at the Laboratory of Biology and Engineering of Cartilage (IBCP)
We are happy to announce that CTE-Lab PhD student Silvia Lopa will spend one month at the Laboratory of Biology and Engineering of Cartilage headed by Prof.
02/05/2012 Meet us at ICRS 2012 in Montreal (12-15 May)!
02/04/2012 Margherita Pierro will spend one month at the Bio-Microsystem Lab (University of Tsukuba)
We are happy to announce that CTE-Lab research fellow Margherita Pierro will spend one month at the Bio-Microsystem Lab headed by Prof. Junji Fukuda (University of Tsukuba, JP).
30/01/2012 Welcome to Nathalie Mayer (University of Lyon)!
We are happy to announce that Nathalie Mayer from University of Lyon (FR) will join our group for 6 months as visiting PhD student.

Pages